Biosurfactant production by pseudomonas aeruginosa strains on 2ml of inoculum size

Objective: to produce biosurfactants from pseudomonas aeruginosa using agricultural resource and to produce biosurfactants using low cost materials. study design: descriptive study place and duration of study: study was conducted at institute of molecular biology and biotechnology in university of lahore. duration of the study was two years. methods: the volume of sample taken are 2ml,of innoculum from growing culture of pseudomonas aeruginosa was isolated from contaminated soil collected from industrial area of district kasoor and flasks were then placed into an orbital shaker at speed of 120rpm. the samples were collected in sterile screw capped bottle,4-5 cm deep from the soil surface aseptically. the samples were stored at 4°c till further use. after every 24h the culture broth from each flask was taken to estimate bacterial cell mass. results: surface tension was 65.8,52.6,47.2 and 33.8 mn/m at time 24,48,72 and 96 hours respectively at constant temperature of 37°c and molasses used 0.25g with 2ml inoculum size. the rhamnolipid production was 0.38,0.97,1.92 and 2.21 g/l respectively. similarly the bacterial cell mass was 0.3,0.65,0.75 and 0.8 g/l respectively conclusion: after optimizing various growth and environmental factors a production of rhamnolipid was achieved.