Isolation and antimicrobial susceptible pattern of bacterial pathogens from ear,nose and throat of paediatric patients

Aim: to investigate the bacterial pathogens of ear,nose and throat (ent). the aim of the study was to determine the frequency and sensitivity pattern of ent infections in paediatric patients.methods: this cross sectional observational study was conducted during july 2012 to december 2012 at the children's hospital and institute of child health,lahore. a total number of 217 samples (172 from ear,11 from nose and 34 from throat) were collected and processed for microbiological investigations. the organisms were identified by routine microbiological techniques. antimicrobial susceptibility profile was determined by modified kirby bauer disc diffusion method.results: out of 217 samples,pathogenic microorganisms were isolated in 136 samples (62.67%) whereas 81(37.33%) samples were culture negative. single organism type was isolated in 121 samples and 15 samples yielded growth of more than one organism. pseudomonas 53(34.87%) was most frequently isolated organism followed by the staphylococcus aureus 34(22.37%). all of the gram positive bacteria were susceptible to vancomycin. majority of gram negative bacteria were susceptible to sulbactam/cefoperazone (96.46%),piperacillin/tazobactam (96.1%),meropenem (93.81%) and amikacin (84.96%).conclusion: this study showed that the pseudomonas species,staphylococcus aureus and klebsiella were the primary pathogens of ent paediatric patients. © 2014 pakistan journal of medical and health sciences. all rights reserved.