Bile bacteriology in patients undergoing cholecystectomy

Background: cholelithiasis is one of the most common surgical and gastroenterological problems worldwide. association of bile flora with formation of gall stones is rarely studied5. keeping in view the importance of bacteriology of bile,our study is focused on the association of bile micro flora with gall stone disease and its postoperative infective complications. methods: study was conducted from august 2011 to december 2013. bile samples were taken from both open and lap cholecystectomies and was sent for culture and sensitivity. data was collected and stores on spss 17. frequencies of different organism were calculated using spss 17 and presented in form of tables and figures. results: total of 80 bile samples were taken and sent for culture. among them 49(61.3%) were female and 31(38.8%) were male patients with mean age of 43.98(sd+/-12.004) years. bile culture of 42(52.5%) did not yield any growth. pseudomonas species were the commonest organism yielding positive culture in 26(32.5%) cases,followed by e coli 7(8.8%),klebshella 4(5%) and enterobacter 1(1.3%). only 2(2.5%) patients were having fever on first post-operative day and 1(1.3%) patient was having ssi. conclusion: association of infective bile with gall stones has been well established in literature,but whether the infected bile is cause of gall stone formation is still debatable. further studies are warranted to establish and strengthen this concept.