Bacterial pathogens in neonatal sepsis and their sensitivity to antibiotics

Aims: to determine the frequency of underlying pathogens in neonatal sepsis and to determine the sensitivity of these pathogens to commonly used antibiotics. place of study: neonatal unit,department of pediatrics,services hospital lahore. study design: it was a cross sectional survey. duration: from: january 2012 to december 2012. method: 100 neonates were enrolled in the study. sample for blood culture was taken under aseptic measures by standardized techniques and were saved in culture bottle. it was collected and transported to the hospital laboratory by single person and inoculate & incubated there. the growth of the pathogen was observed for next 5 days and sensitivity pattern was checked by using different antibiotic discs. culture and sensitivity pattern as shown by blood culture report was noted. results: frequency of bacterial pathogens were 33(33%) klebsiella,25(25%) pseudomonas,19(19%) e.coli and staphylococcus 23(23%),while frequency of antimicrobial sensitivity reveals 79% ciprofloxacin,81% amikacin,17% penicillin and 11% gentamicin for staphylococcus aureus. sensitivity for pseudomonas was 56% ciprofloxacin,59% amikacin,1% penicillin,imipenum (79%) and 35% gentamicin. for pathogens klebsiella it was 43% ciprofloxacin,33% amikacin,and 8% impineum while ciprofloxacin 47%,amikacin 52% and imipenum 76% for e.coli. conclusion: we concluded that klebsiella and pseudomonas are the leading pathogens in neonatal sepsis and the most commonly used antibiotics using in our setup are highly resistant with them which may be used when culture reports are awaiting.