Cloning of a β-glucosidase gene from thermophilic fungus Cheatomium thermophilum

The investigation was focused on cellulolytic enzymes from cheatomium thermophilum,a thermophilic fungus that produces cellulose degrading enzymes and has been exploited by the industry. it was found that the fungus showed maximum growth on eggins and pugh medium at ph 5 and by using carboxymethyl cellulose (cmc) as carbon source. rna was isolated from mycelia of cheatomium thermophilum and cdna was synthesized. the subsequent pcr products were purified through agarose gel electrophoresis and ligated into puc19. the recombinant vectors containing the bgl gene were transformed into e coli dh10b for further characterization.