|
|
|
|
Bacillus subtilis improvement through UV and chemical mutagenesis for indigenously hyperproduced urate oxidase
|
|
|
|
|
|
|
|
نویسنده
|
meraj m. ,khalil-ur-rahman department of chemistry and biochemistry ,jamil a. ,ashraf m. ,rajoka m.i. ,javed s. ,jahan n.
|
|
منبع
|
pakistan journal of life and social sciences - 2012 - دوره : 10 - شماره : 2 - صفحه:123 -129
|
|
چکیده
|
The main purpose of this research was to optimize the production of urate oxidase through mutagenesis of bacillus subtilis. the organism was subjected to ultra violet irradiation and chemical mutagenesis. the strain was chemically mutated by using ethyl methane sulfonate and ethidium bromide. 2- thiouric acid was used as a selective marker to identify the uv and chemically mutated bacillus subtilis. ethyl methane sulfonate treated b. subtilis (180 minutes dose) was proved to be the best for optimum production of urate oxidase by 3 log kill/survival curve. fermentation medium was also optimized,it was found that substrate concentration (0.5%),fermentation period (36 h),ph (8.5),temperature (35°c),inoculum size (3%) enhanced the activity (42.77±0.36 u/ml) of mutant derived enzyme.
|
|
کلیدواژه
|
Bacillus subtilis; Ethyl methane sulfonate; Fermentation medium; Mutagenesis; Urate oxidase
|
|
آدرس
|
department of chemistry and biochemistry,university of agriculture, Pakistan, university of agriculture, Pakistan, department of chemistry and biochemistry,university of agriculture, Pakistan, department of botany,university of agriculture, Pakistan, department of chemistry,government college university, Pakistan, department of chemistry,government college university, Pakistan, department of chemistry and biochemistry,university of agriculture, Pakistan
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Authors
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|