Hepatitis B virus Genotypes from Clinical Sample of Hepatitis B Antigen Positive Patients by Using PCR Method in Kohat Region of Khyber Pakhtunkhwa,Pakistan

Hepatitis b is an infection of hepatitis b virus which is the burning health problem in khyber pakhtunkhwa causing high morbidity and mortality. a total of 84 hbsag samples were subjected to pcr for dna detection and genotyping. of them,75 (89.28%) were found hbv dna positive and 9 (10.71%) were negative. the highest prevalence was found in the group 31-40 years (33.33%) followed by the group 21-30 years (29.76%),41-50 years (21.42%) and above 51 years (15.47%). the lowest prevalence was found in the group <20 years (0%). in risk factors,the blood transfusions 25.00%,surgery 19.04% and hospitalization were 22.61% in the total population. in case of hygienic conditions,the community belonging to poor hygienic conditions was found to be more frequently infected 67.85% as compared to the good (32.14%). genotype a was most common in all age groups while c,d were comparatively less frequent. males were found to be more frequently infected (69.33%) as compared to females (30.66%) with a positivity ratio of 2.26: 1,respectively. it is concluded that genotype a,c,d and f are the most common genotypes in kohat region of khyber pakhtunkhwa. pcr is more sensitive and specific method for hbv detection.