Detection and sequencing of field isolates of peste des petits ruminants virus from punjab province,Pakistan

Peste des petits ruminants (ppr),is a viral contagious and important transboundary animal disease (tad),affecting domestic and wild small ruminants like sheep and goats,endemic in indo-pak subcontinent. the study was undertaken to characterize the indigenous pprv by characterizing the fusion protein (f) gene segments by sequencing and their phylogenetic analysis for determination of genetic variation in the field viruses. selected f gene segments of pprv genome were amplified using rt-pcr from 110 tissues (lungs,lymph nodes and spleen) samples from dead animals and 41 swabs (nasal,occular and oral) from ppr suspected animals. the resulting amplicons were sequenced for phylogenetic analysis. the phylogenetic tree based on the f gene sequences of pprv from different locations clustered them into lineage 4 along with the other isolates from pakistan. thus,genetic diversity of pprv into the same lineages based on the f gene sequences showed the presence of lineage 4 emerged to give up the thought of molecular epidemiology for pprv.