Effect of saline growth medium on temperature optima of invertases in sugarcane (Saccharum officinarum L.)

Sugarcane is a major source of sugar production in pakistan but high levels of salts present in the soil reduce its growth considerably. therefore,physiological and biochemical basis of yield reduction under saline conditions was studied. invertases were extracted,purified and characterized from sugarcane grown under salinity to evaluate the effect of saline conditions on various characteristics of these enzymes. an attempt was made to investigate the possible effect of salinity on the stability of soluble acid invertases (sai) in terms of temperature optima from two local cultivars of sugarcane. the temperature optimum of native sai of cp-77-400 and coj-84 was found to be 55 and 50°c respectively. the saline growth medium did not affect temperature optima of coj-84 invertases,while sai from cp-77-400 showed a 5 degree decrease at all salinity levels.