Investigations of foot-and-mouth disease outbreaks in Faisalabad district of Punjab,Pakistan during the year 2013

Sampling was done from various outbreaks of foot-and-mouth disease (fmd) that occurred during the year 2013 in district faisalabad,punjab,pakistan. reverse transcriptase-polymerase chain reaction (rt-pcr) was used for typing of foot-and-mouth disease virus (fmdv). a total of 110 clinical samples were received from different administrative regions of faisalabad; 86 were found fmdv genome positive when tested with 1f and 1r consensus primers. out of these genome positive samples,73 were successfully typed into serotypes a (19 samples),o (30 samples) and asia1 (24 samples). complementary dnas (cdnas) were stored at - 80°c for further sequencing studies. serotype a fmdv was detected from samples received from outbreaks that occurred in sumandri,jaranwala and tandlianwala tehsils,serotype o from tandlianwala and sadhar,and serotype asia 1 outbreaks were reported from chak jhumra and sadhar.