Efficacy of azadirachta indica and solanum nigrum for skin regeneration in mice

Skin is a protective shield of body against external hazards of environment which can interfere with its structural and metabolic mechanism. consequences of any chemical or physical injury integrity are significant in medical sciences. rate of skin recovery and regeneration indirectly ensures the revitalization of internal normal functioning of body. in the present animal model,ethanolic extracts of two local herbs,azadirecta indica and solanum nigrum were applied for skin wounds healing up to 4 weeks. male mature mice were grouped as intact control,experimental control,a (a. indica+ glycerin treated),b (s. nigrum + glycerin treated),c (a. indica + s. nigrum treated) and d (glycerin treated). histopathological results revealed the controlled microbial attacks in groups a and b,as compared to the wounded sites of groups c and d. re-epithelialization and granulation rates were noted higher in group b after 1st week of wound induction. significant regeneration of hair follicles and reinnervation in group b were recorded with mean diameters 6l63μm and 57.13μm,respectively,at first week of following wound induction. similarly,angiogenesis rate was found better in group b with capillaries' average diameter of 14.25μm at 1st week and this re-growth delayed till 2nd week for other experimental groups. moreover,in morphological examination,it was noticed that body weight steadily increased during the study period for the group a; a trend which was not observed for the other experimental groups. wound surface area also reduced quickly down to 187.5μm2 in group a during at 1st week of post trauma. hematological analysis showed that ratios of direct leukocyte count and platelets were less in blood smears of groups a,b and c than in experimental control and group d. results of the present study recommend that low cost local and natural flora should be preferred over harmful synthetic produce to make stimulatory drugs for enhanced skin wound healing and with better regeneration rate accompanied with minimum side effects.