bioremediation of phenanthrene-polluted soil using bacillus kochii ahv-kh14 as a halo-tolerant strain isolated from compost

Background: phenanthrene (phe) is a polycyclic aromatic hydrocarbon (pah) with crystalline structure of c14h10, which was produced from incomplete combustion of hydrocarbons and fossil fuels and can cause harmful biological effects. bioremediation using halophilic bacteria is payed attention over chemical methods due to considerable benefits.methods: in the present study, a halotolerant bacterium bacillus kochii strain ahvkh14 was isolated from municipal compost, and used for the bioremediation of phe from the contaminated soil. the effects of operational parameters including soil/water ratio, initial inoculum size, phe concentration, and salinity on the bioremediation performance were investigated.results: a biodegradation efficiency of about to 98% was obtained for phe concentration of 50 mg/kg and salinity level of 1.5%. by increasing salinity content phe concentration, phe biodegradation rate decreased significantly. it was found that the bioremediation efficiency decreased with increasing phe concentration. it was also revealed that for the unwashed soil sample, cumulative concentrations of different hydrocarbons played an important role in decreasing the efficiency of bioremediation.conclusion: a natural hydrocarboncontaminated soil sample with total petroleum hydrocarbon (tph) concentration of 2350 mg/kg was subjected to bioremediation using the selected conditions of operational parameters, and a biodegradation rate of 17.7% was obtained.