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Comparative study on molecular weight of konjac glucomannan by gel permeation chromatography-laser light scattering-refractive index and laser light-scattering methods
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نویسنده
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xu m. ,li d.-s. ,li b. ,wang c. ,zhu y.-p. ,lv w.-p. ,xie b.-j.
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منبع
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journal of spectroscopy - 2013 - دوره : 2013 - - کد همایش:
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چکیده
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The gel permeation chromatography,combined with laser light scattering and refractive index (gpc-lls-ri) online method and laser light scattering (lls) single method,researched the weight-average molecular weight (mw) of konjac glucomannan (kgm) in this paper. the results show that the determination results of molecular weight of kgm are similar by two method,the molecular weight of kgm is 2.476 × 10 5 g/mol by online method measured,and it is 2.508 × 10 5 g/mol by single method measured,compared with only 1.3% difference between the two methods. at the same time,the molecular weight distributions of kgm and conformation index were analyzed by online method,while the second virial coefficient (a 2) that characterizes the interaction between kgm molecules and solvent molecules was acquired by single method,which can provide reliable foundation for the molecular chain morphology research of kgm. © 2013 mei xu et al.
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آدرس
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key laboratory of fermentation engineering,ministry of education,hubei university of technology,wuhan 430068,china,research center of food fermentation engineering and technology of hubei, China, key laboratory of fermentation engineering,ministry of education,hubei university of technology,wuhan 430068,china,research center of food fermentation engineering and technology of hubei, China, college of food science and technology,huazhong agricultural university, China, key laboratory of fermentation engineering,ministry of education,hubei university of technology,wuhan 430068,china,research center of food fermentation engineering and technology of hubei, China, key laboratory of fermentation engineering,ministry of education,hubei university of technology,wuhan 430068,china,research center of food fermentation engineering and technology of hubei, China, key laboratory of fermentation engineering,ministry of education,hubei university of technology,wuhan 430068,china,research center of food fermentation engineering and technology of hubei, China, college of food science and technology,huazhong agricultural university, China
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Authors
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