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Assessment of the inhibitory effect of rifampicin on amyloid formation of hen egg white lysozyme: Thioflavin T fluorescence assay versus FTIR difference spectroscopy
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نویسنده
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ma g. ,zhang h. ,guo j. ,zeng x. ,hu x. ,hao w.
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منبع
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journal of spectroscopy - 2014 - دوره : 2014 - شماره : 0
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چکیده
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The inhibitory effect of rifampicin on the amyloid formation of hen egg white lysozyme was assessed with both thioflavin t (tht) fluorescence assay and fourier transform infrared (ftir) difference spectroscopy. we reveal that tht fluorescence assay gives a false positive result due to rifampicin interference,while ftir difference spectroscopy provides a reliable assessment. with ftir,we show that rifampicin only has marginally inhibitory effect. we then propose that ftir difference spectroscopy can potentially be a convenient method for inhibitor screening in amyloid study. © 2014 gang ma et al.
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آدرس
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key laboratory of medicinal chemistry and molecular diagnosis of ministry of education,college of chemistry and environmental science,hebei university, China, key laboratory of medicinal chemistry and molecular diagnosis of ministry of education,college of chemistry and environmental science,hebei university, China, key laboratory of medicinal chemistry and molecular diagnosis of ministry of education,college of chemistry and environmental science,hebei university, China, key laboratory of medicinal chemistry and molecular diagnosis of ministry of education,college of chemistry and environmental science,hebei university, China, key laboratory of medicinal chemistry and molecular diagnosis of ministry of education,college of chemistry and environmental science,hebei university, China, key laboratory of medicinal chemistry and molecular diagnosis of ministry of education,college of chemistry and environmental science,hebei university, China
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Authors
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