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Interaction between chromosomal protein hmgb1 and DNA studied by DNA-melting analysis
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نویسنده
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chikhirzhina e.v. ,tatiana s.j. ,polyanichko a.m.
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منبع
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journal of spectroscopy - 2014 - دوره : 2014 - شماره : 0
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چکیده
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Interaction of hmgb1 nonhistone chromosomal protein with dna was studied using circular dichroism spectroscopy and thermal denaturation of dna. melting dna in the complex was shown to be a biphasic process. the characteristic melting temperatures of unbound dna and the dna bound to hmgb1 in 0.25 mm edta solutions were found to be tmi=44.0±0.5°c and tmii=62.0±1°c,respectively. it was shown that the binding of the hmgb1 molecule affects the melting of the dna region approximately 30 b.p. long. © 2014 elena v. chikhirzhina et al.
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آدرس
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institute of cytology,russian academy of sciences, Russian Federation, institute of cytology,russian academy of sciences,saint petersburg,russian federation,department of molecular biophysics,faculty of physics,saint petersburg state university,1 ulyanovskaya street,stary petergoff, Russian Federation, institute of cytology,russian academy of sciences,saint petersburg,russian federation,department of molecular biophysics,faculty of physics,saint petersburg state university,1 ulyanovskaya street,stary petergoff, Russian Federation
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Authors
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