Simultaneous determination of four compounds in a nelumbo nucifera seed embryo by HPLC-DAD

Nelumbo nucifera has a variety of biological activities. so it was importantly used as various herbal medicines since traditional times. a simple,fast,and sensitive high-performance liquid chromatographic (hplc) method was developed in this study for efficient quality control of n. nucifera. four different compounds,including neferine,1,2,3,4-tetrahydro-1-[(4-hydroxyphenyl) methyl]-2-methyl-7-isoquinolinol,1-hydroxy-2-methylpropene,and 3-(prop-1-enyl)benzene-1,2,4,5-tetrol,were simultaneously determined. the four compounds were isolated through a dionex c18 column by gradient elution with 0.1% tfa-water and methanol. the flow rate was 1.0 ml/min,and the wavelength was detected at 205,254,280,and 330 nm. the chromatograms were acquired at 205 nm. the four compounds showed good linear relationships (r2>0.96) over five different concentrations,and an average recovery of the method ranged from 96.27% to 108.78%. through the analysis validation test and application of the method,the optimized conditions verified that it is efficient to isolate the compounds of n. nucifera seed embryos. © 2017 gahee ryu et al.