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Efficient and rapid detection of salmonella using microfluidic impedance based sensing
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نویسنده
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ghosh dastider s. ,barizuddin s. ,yuksek n.s. ,dweik m. ,almasri m.f.
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منبع
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journal of sensors - 2015 - دوره : 2015 - شماره : 0
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چکیده
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We present a low cost,easy to fabricate biosensor,which can quickly and accurately detect salmonella typhimurium. this study also compares the advantages of the microfluidic biosensor over a nonmicrofluidic biosensor. high density interdigitated electrode array was used to detect salmonella cells inside a microfluidic chip. monoclonal anti-salmonella antibodies were allowed to be immobilized on the surface of the electrode array for selective detection of salmonella typhimurium. an impedance analyzer was used to measure and record the response signal from the biosensor. the biosensor provides qualitative and quantitative results in 3 hours without any enrichment steps. the microfluidic biosensor's lower detection limit was found to be 3 × 10 3 cfu/ml compared to the 3 × 10 4 cfu/ml of the nonmicrofluidic biosensor,which shows that the microfluidic biosensor has 10-fold increased sensitivity. the impedance response of microfluidic biosensor was also significantly higher (2 to 2.9 times) compared to the nonmicrofluidic biosensor. © 2015 shibajyoti ghosh dastider et al.
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آدرس
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department of electrical and computer engineering,university of missouri,columbia, United States, cooperative research and life and physical sciences,lincoln university,jefferson city, United States, department of electrical and computer engineering,university of missouri,columbia, United States, cooperative research and life and physical sciences,lincoln university,jefferson city, United States, department of electrical and computer engineering,university of missouri,columbia, United States
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Authors
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