Bioluminescence-sensing assay for microbial growth recognition

The conventional methods for microbial viability quantification require cultivation and are laborious. there is consequently a widespread need for cultivation-free methods. the adenosine triphosphate (atp) bioluminescence-sensing assay is considered an extremely effective biosensor; hence atp is the energy currency of all living microbes and can be used as a rapid indicator of microbial viability. we developed an atp bioluminescence-sensing assay to detect microbial viability. a bioluminescent recombinant e. coli strain was used with luciferase extracted from transformed bacteria. results showed that there is a direct correlation between the bioluminescence intensity of the atp bioluminescence-sensing assay and the microbial viability. bacterial counts from food samples were detected using the developed sensing assay and validated by the traditional plate-counting method. compared with the plate-counting method,atp bioluminescence-sensing assay is a more rapid and efficient approach for detecting microbial viability. © 2016 heba ramadan eed et al.