Detection of Stress Hormone in the Milk for Animal Welfare Using QCM Method

We developed a rapid and sequential analysis system to determine stress marker in the milk. one of the famous stress markers,cortisol,was detected using our method. quartz crystal microbalance (qcm) method with a twin sensor was used in this study. one channel detected stress marker corresponding to antigen-antibody interaction and the other channel was used as a reference to remove environmental influences. although nonspecific adsorption was monitored on each channel,frequency difference between them was within a few hz on the injection of sample solution. one determination cycle including regeneration step could be performed within 10 minutes. the system could detect the cortisol level from 0.1 pg/ml to 100 pg/ml. these results show that our system has a potential to check the daily feeding condition for cows in terms of animal welfare. © 2017 takeshi ito et al.