Advanced corneal cross-linking system with fluorescence dosimetry

Purpose. this paper describes an advanced system that combines corneal cross-linking with riboflavin with fluorescence dosimetry,the ability to measure riboflavin diffusion within the cornea both before and during uva treatment. methods and results. a corneal cross-linking system utilizing a digital micromirror device (dmd) was assembled and used to measure diffusion coefficients of 0.1% riboflavin in 20% dextran in porcine eyes. a value of (3.3±0.2)×10-7 cm2/s was obtained for the stroma. diffusion coefficients for the transepithelial formulation of 0.1% riboflavin in 0.44% saline and 0.02% bak were also measured to be 4.7±0.3×10-8 cm2/s for epithelium only and (4.6±0.4)×10-7 cm 2/s for stroma only. riboflavin consumption during a uva treatment was also demonstrated. conclusion. a new advanced corneal cross-linking system with fluorescence dosimetry of riboflavin has been demonstrated. it is hoped that this method may play a significant role in determining the underlying mechanisms of corneal cross-linking and assist with the development of additional riboflavin formulations. moreover,dosimetry may prove valuable in providing a method to account for the biological differences between individuals,potentially informing cornea-specific uva treatment doses in real time. © 2012 marc d. friedman et al.