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RNA Interference targeting connective tissue growth factor inhibits the transforming growth factor- β 2 induced proliferation in human Tenon capsule fibroblasts
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نویسنده
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jing j. ,li p. ,li t. ,sun y. ,guan h.
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منبع
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journal of ophthalmology - 2013 - دوره : 2013 - شماره : 0
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چکیده
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Purpose. this study was to determine the effect of ctgf-small interfering rna (sirna) on tgf-β2-induced proliferation in human tenon capsule fibroblasts (htfs). methods. htfs were transfected with four of ctgf-sirnas separately for screening of gene silencing efficacy that was determined by transcript level measured by quantitative real-time pcr (qrt-pcr). recombinant tgf-β2 was added into the culture to stimulate the proliferation of htfs. the gene silencing efficacy of the sirnas was evaluated by qrt-pcr and immunofluorescence of ctgf transcript and protein levels. the viability of htfs was determined by cell counting kit-8 (cck-8). fcm was used to assess cell cycle after ctgf-sirna transfection. results. the expression of ctgf and proliferation of htfs were increased significantly by tgf-β2 stimulation. the transfection of ctgf-sirna abolished the upregulation of ctgf and cell proliferation induced by tgf-β2. the analysis of cell cycle indicated that ctgf-sirna treatment stimulated cells from s phase to g0/g1 phase in comparison with the inverse physiologic function of tgf-β2. conclusion. ctgf targeting sirna could effectively suppress the expression of ctgf and attenuate the proliferation of htfs. the sirna approach may provide a therapeutic option for eliminating filtration bleb scarring after glaucoma filtration surgery (gfs). © 2013 jiaona jing et al.
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آدرس
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eye institute,affiliated hospital of nantong university,20 xisi road,nantong,jiangsu province 226001,china,nanjing governmental hospital,116 chengxian street,nanjing, China, eye institute,affiliated hospital of nantong university,20 xisi road,nantong, China, department of life science center,biomics biotechnologies co.,ltd.,eandt development area,76 changxing road,nantong,jiangsu province 226016,china,small rna technology and application institute,nantong university,eandt development area,76 changxing road,nantong, China, department of life science center,biomics biotechnologies co.,ltd.,eandt development area,76 changxing road,nantong,jiangsu province 226016,china,small rna technology and application institute,nantong university,eandt development area,76 changxing road,nantong, China, eye institute,affiliated hospital of nantong university,20 xisi road,nantong, China
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Authors
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