Expression of Wnt/ β -Catenin Signaling Pathway and Its Regulatory Role in Type i Collagen with TGF- β 1 in Scleral Fibroblasts from an Experimentally Induced Myopia Guinea Pig Model

Background. to investigate wnt/β-catenin signaling pathway expression and its regulation of type i collagen by tgf-β1 in scleral fibroblasts from form-deprivation myopia (fdm) guinea pig model. methods. wnt isoforms were examined using genome microarrays. scleral fibroblasts from fdm group and self-control (sc) group were cultured. wnt isoforms,β-catenin,tgf-β1,and type i collagen expression levels were examined in the two groups with or without dkk-1 or tgf-β1 neutralizing antibody. results. for genome microarrays,the expression of wnt3 in fdm group was significantly greater as confirmed in retinal and scleral tissue. the expression of wnt3 and β-catenin significantly increased in fdm group and decreased significantly with dkk-1. tgf-β1 expression level decreased significantly in fdm group and increased significantly with dkk-1. along with morphological misalignment inside and outside cells,the amount of type i collagen decreased in fdm group. furthermore,type i collagen increased and became regular in dkk-1 intervention group,whereas it decreased and rearranged more disorder in tgf-β1 neutralizing antibody intervention group. conclusions. the activation of wnt3/β-catenin signaling pathway was demonstrated in primary scleral fibroblasts in fdm. this pathway further reduced the expression of type i collagen by tgf-β1,which ultimately played a role in scleral remodeling during myopia development. � 2016 min li et al.