Effect of MT3 on Retinal and Choroidal TGF-β2 and HAS2 Expressions in Form Deprivation Myopia of Guinea Pig

Purpose. to confirm its dose-dependent effect on form deprivation myopia and evaluate the effect of mt3 at different tissue concentrations on changes in mrna and protein expression for tgf-β2 and has2. methods. mt3 was intravitreally injected into deprived eyes at two-day intervals. refraction was measured by streak retinoscopy after cycloplegia. the axial dimensions were measured by a-scan ultrasound. the quantitative rt-pcr and western blot were used to detect the changes of tgf-β2 and has2 expressions in the retina and choroid of guinea pigs. results. mt3 treatment produced a significant dose-dependent reduction in relative myopia compared to fd group (both p<0.001). there were statistically significant increases in retinal and choroidal mrna levels for both tgf-β2 and has2 after injections of 10 μm of mt3,when compared to the fd group. there were no significant differences in retinal and choroidal tgf-β2 protein expression levels between the mt3 treatment groups and fd group (all p>0.05). the injections of 10 μm of mt3 caused a marked decrease in retinal has2 protein expression level,when compared to the fd group (p=0.001). conclusion. mt3 can inhibit form deprivation myopia,and mt3 treatment can result in changes of retinal and choroidal tgf-β2 and has2 mrna and protein expressions. © 2017 tao li et al.