>
Fa   |   Ar   |   En
   A Critical Analysis of the Available in Vitro and Ex Vivo Methods to Study Retinal Angiogenesis  
   
نویسنده moleiro a.f. ,conceição g. ,leite-moreira a.f. ,rocha-sousa a.
منبع journal of ophthalmology - 2017 - دوره : 2017 - شماره : 0
چکیده    Angiogenesis is a biological process with a central role in retinal diseases. the choice of the ideal method to study angiogenesis,particularly in the retina,remains a problem. angiogenesis can be assessed through in vitro and in vivo studies. in spite of inherent limitations,in vitro studies are faster,easier to perform and quantify,and typically less expensive and allow the study of isolated angiogenesis steps. we performed a systematic review of pubmed searching for original articles that applied in vitro or ex vivo angiogenic retinal assays until may 2017,presenting the available assays and discussing their applicability,advantages,and disadvantages. most of the studies evaluated migration,proliferation,and tube formation of endothelial cells in response to inhibitory or stimulatory compounds. other aspects of angiogenesis were studied by assessing cell permeability,adhesion,or apoptosis,as well as by implementing organotypic models of the retina. emphasis is placed on how the methods are applied and how they can contribute to retinal angiogenesis comprehension. we also discuss how to choose the best cell culture to implement these methods. when applied together,in vitro and ex vivo studies constitute a powerful tool to improve retinal angiogenesis knowledge. this review provides support for researchers to better select the most suitable protocols in this field. © 2017 a. f. moleiro et al.
آدرس departamento de cirurgia e fisiologia,faculdade de medicina,universidade do porto,porto,portugal,unidade de investigação cardiovascular,faculdade de medicina,universidade do porto,porto, Portugal, departamento de cirurgia e fisiologia,faculdade de medicina,universidade do porto,porto,portugal,unidade de investigação cardiovascular,faculdade de medicina,universidade do porto,porto, Portugal, departamento de cirurgia e fisiologia,faculdade de medicina,universidade do porto,porto,portugal,unidade de investigação cardiovascular,faculdade de medicina,universidade do porto,porto,portugal,departamento de cirurgia cardiotorácica,centro hospitalar são joão,porto, Portugal, departamento de cirurgia e fisiologia,faculdade de medicina,universidade do porto,porto,portugal,unidade de investigação cardiovascular,faculdade de medicina,universidade do porto,porto,portugal,departamento de cirurgia cardiotorácica,centro hospitalar são joão,porto,portugal,departamento de oftalmologia,centro hospitalar são joão,porto, Portugal
 
     
   
Authors
  
 
 

Copyright 2023
Islamic World Science Citation Center
All Rights Reserved