Effects of the Rho-Kinase Inhibitor Y-27632 on Extraocular Muscle Surgery in Rabbits

Purpose. to evaluate the effect of the rho-kinase inhibitor y-27632 on postoperative inflammation and adhesion following extraocular muscle surgery in rabbits. methods. the superior rectus muscle reinsertion was performed on both eyes of 8 new zealand white rabbits. after reinsertion,the rabbits received subconjunctival injections of the rho-kinase inhibitor and saline on each eye. to assess acute and late inflammatory changes,ki-67,cd11β+,and f4/80 were evaluated and the sites of muscle reattachment were evaluated for a postoperative adhesion score and histopathologically for collagen formation. results. f4/80 antibody expression was significantly different in the rho-kinase inhibitor-injected group at both postoperative day 3 and week 4 (p=0.038,0.031). however,ki-67 and cd11β+ were not different the between two groups. the difference in the srm/conjunctiva adhesion score between the two groups was also significant (p=0.034). conclusion. intraoperative subconjunctival injection of the rho-kinase inhibitor may be effective for adjunctive management of inflammation and fibrosis in rabbit eyes following extraocular muscle surgery. © 2017 ji-sun moon et al.