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   Reliability of a Fully Automated Interpretation of γ -H2AX Foci in Lymphocytes of Moderately Trained Subjects under Resting Conditions  
   
نویسنده heydenreich j. ,otto c. ,mayer f. ,carlsohn a.
منبع journal of nutrition and metabolism - 2014 - دوره : 2014 - شماره : 0
چکیده    Background. analysis of γ-h2ax foci is a promising approach to evaluate exercise-induced dna damage. however,baseline levels and day-to-day variability of γ-h2ax foci have not been investigated in healthy subjects at rest. methods. blood was taken from eight moderately trained healthy males (29 ± 3 yrs,1.84 ± 0.03 m,and 85 ± 6 kg) at two separate days (m1/m2) after 24-hour exercise cessation. number of γ-h2ax foci per 100 lymphocytes (n),number of foci per affected lymphocyte (nal),percentage of affected lymphocytes (pal),and diameter (d) of γ-h2ax foci were analyzed (mean ± sd). differences between m1 and m2 were analyzed using paired t-tests (0.05). day-to-day variability was evaluated by calculating the coefficients of variation (cv%),bias,and limits of agreement (loa). results. there were no statistically significant differences between m1 (n: 7.6 ± 4.4,nal: 1.2 ± 0.2,pal: 5.9 ± 2.6%,and d: 0.63 ± 0.07) and m2 (n: 8.4 ± 4.6,nal: 1.3 ± 0.1,pal: 6.9 ± 4.2%,and d: 0.66 ± 0.06). cv was calculated to be 98.5% (n),88.9% (pal),11.3% (nal),and 8.0% (d). bias (loa) was 0.75 (-15.2/13.7),-0.02 (-0.36/0.33),-1.0 (-11.9/9.9),and -0.04 (-0.16/0.09),respectively. conclusions. background level in healthy subjects is approximately 0.07 to 0.09 γ-h2ax foci/cell. nal and d are reliable measures. © 2014 juliane heydenreich et al.
آدرس sports medicine and sports orthopaedics,university outpatient clinic,university of potsdam,potsdam,germany,ziel-research centre for nutrition and food sciences,munich technical university, Germany, sports medicine and sports orthopaedics,university outpatient clinic,university of potsdam, Germany, sports medicine and sports orthopaedics,university outpatient clinic,university of potsdam, Germany, sports medicine and sports orthopaedics,university outpatient clinic,university of potsdam,potsdam,germany,institute of health science,schwäbisch gmünd university of education, Germany
 
     
   
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