Inhibition of Intestinal α -Glucosidase and Glucose Absorption by Feruloylated Arabinoxylan Mono- and Oligosaccharides from Corn Bran and Wheat Aleurone

The effect of feruloylated arabinoxylan mono- and oligosaccharides (faxmo) on mammalian α-glucosidase and glucose transporters was investigated using human caco-2 cells,rat intestinal acetone powder,and xenopus laevis oocytes. the isolated faxmo from wheat aleurone and corn bran were identified to have degree of polymerization (dp) of 4 and 1,respectively,by hplc-ms. both faxmo extracts were effective inhibitors of sucrase and maltase functions of the α-glucosidase. the ic50 for faxmo extracts on caco-2 cells and rat intestinal α-glucosidase was 1.03-1.65 mg/ml and 2.6-6.5 mg/ml,respectively. similarly,glucose uptake in caco-2 cells was inhibited up to 40%. the inhibitory effect of faxmo was dependent on their ferulic acid (fa) content (r = 0.95). sodium independent glucose transporter 2 (glut2) activity was completely inhibited by faxmo in oocytes injected to express glut2. our results suggest that ferulic acid and feruloylated arabinoxylan mono-/oligosaccharides have potential for use in diabetes management. © 2016 lovemore nkhata malunga et al.