Plasma and Aorta Biochemistry and MMPs Activities in Female Rabbit Fed Methionine Enriched Diet and Their Offspring

This study investigated whether a high met diet influences biochemical parameters,mmps activities in plasma,and biochemical and histological remodeling in aorta,in both pregnant female rabbits and their offspring. four female rabbit groups are constituted (each n=8),nonpregnant control (npc),pregnant control (pc) that received normal commercial chow,nonpregnant met (npmet),and pregnant met (pmet) that received the same diet supplemented with 0,35% l-methionine (w/w) for 3 months (500 mg/d). all pregnant females realize 3 successive pregnancies. plasma results showed that met excess increased hcy,raised crp in npmet and decreased it in pmet,enhanced significantly prommp-2 and prommp-9 activities in npmet,and reduced them in pmet. aorta showed a rise in collagen level,essentially in pmet,a reduction of elastin content in both pmet and npmet,and a significant decrease in lipid content in pmet,with histological changes that are more pronounced in npmet than pmet. met excess enhanced prommp-9 activities in npmet while it decreased them in pmet. pmet newborn presented increase in uremia and crp and significant rise of active mmp-2 and mmp-9 forms. in aorta,media and adventitia thickness increased,total lipids content decreased,prommp-9 activity decreased,and prommp-2 activity increased. � 2017 khira othmani mecif et al.