Differential expression of vimentin and GFAP protein during brain development of mouse fetuses after treated with 2-methoxyethanol

2-methoxyethanol is known as a chemical pollutant that is teratogenic or embryotoxic in animals and humans. 2-me and its metabolites can damage cells and tissues in the body. investigation of protein expression due to 2-me was performed using primary antibodies vimentin and gfap. the amount of protein expressed was measured by immunodot blot and densitometry techniques. the results show that the average total change in vimentin protein levels was higher (22.24% at gestation day 17 and 18) than in gfap protein levels (15.10% on gestation day 12) in the treatment group. the 2-me disrupted translation of vimentin and increased the level of gfap expression due to the cellular response to toxic materials. it can be concluded that the vimentin protein is essential for the development of the brain,especially to stimulate cell proliferation for cell repair. © 2012 published by lppm itb.