First identification of a novel probiotic bacterium Streptococcus phocae and it's beneficial role in diseases control

The strain pi80 which showed promising antimicrobial effect on different shrimp and fish pathogens was isolated from gut of shrimp penaeus indicus. based on 16sr rna gene sequence similarities and phenotypic characterization,strain pi80 was identified as a member of the genus streptococcus. the g+c content of the partial dna sequence was 53 %. streptococcus phocae pi80 exhibited resistance against methicillin,kanamycin,neomycin and amikacin. hemolytic and phenotypic results showed the differentiation of strain pi80 from other species within the genus streptococcus. s. phocae pi80 showed broad spectrum of antagonistic activity against gram-positive and gram-negative pathogens by producing its own antimicrobial compound like bacteriocin. moreover the culture growth parameters revealed that the pi80 having potential to grow at high temperature,ph and nacl concentration.