Formulation and Evaluation of new biodegradable periodontal chips from Malaysian propolis in chitosan base

This study aims to formulate periodontal chips from malaysian propolis in chitosan base and to evaluate the physical,biological and antibacterial properties. the propolis was extracted using ultrasonic method,diluted and mixed with chitosan to produce the propolis chips. thickness and morphology of the chips were evaluated using sem,the roughness was measured using profilometer. the chips were tested for biodegradability towards trypsin. absorption test was done on artificial saliva and distilled water. in vitro release of propolis was evaluated by using spectrophotometer for 15 days. antibaceterial effects of the propolis were analysed using minimal inhibitory concentration and minimal bactericidal concentration methods for gram positive and gram negative bacteria. propolis chips showed complete biodegredation on day 14. the release of propolis was up to 80% at day 6 and continued to increase within the experimental period. mic values of extracted propolis,for gram positive and gram negative bacteria were found to be 0.15mg/ml and 1.25mg/ml respectively. while for the mbc the value was found to be 0.31 mg/ml and 0.25 mg/ml respectively. propolis chips were found to be biodegradable,exhibiting a high release rate and possessing antibacterial effects towards gram positive and gram negative bacteria.