Quantification of Lipot eichoic acid of gram positive bacteria after irrigation with sodium hypochlorite in root canal-an in vitro study

Bacteria play an important role in root canal infection. lipot eichoic acid (lta) is the one of virulence factor released by gram positive bacteria. the aim of this study was to quantify the lta levels after irrigation with sodium hypochlorite. seventy two single rooted mandibular premolar tooth was taken for the study. gram positive and negative bacteria were grown in these teeth. teeth were then irrigated with sodium hypochlorite. the dentinal shavings were collected for lta quantification. sodium hypochlorite irrigated in tooth with gram positive bacteria showed significant increase in lta levels(p < 0.05) gram positive bacteria alone showed increase in lta levels than that of combination of gram positive,negative bacteria,after sodium hypochlorite disinfection.