Antibacterial efficacy of 5% Ethanolic extract of propolis (EEP) solution against enterococcus faecalis (laboratory experiment)

2% chlorhexidine is proven to be effective against enterococcus faecalis (e. faecalis),however chlorhexidine is known to be toxic against several particular cells. propolis is a non-toxic natural resinous substance. furthermore,5% ethanolic extract of propolis (eep) solution is proven to be effective against e. faecalis. the aim of this study is to investigate the comparison of the antibacterial efficacy of 5% eep solution and 2% chlorhexidine solution against e. faecalis. 24 tubes were divided into 4 groups. on group 1 and 2,the tubes were filled with 1 ml of bhi suspension inoculated by e. faecalis and 1ml of the tested solutions. on group 3 and 4,the tubes were filled with 2 ml of bhi suspension,with and without e. faecalis,as control groups. all tubes were then incubated at 37°c for 24 hours. the value of optical density (od) was measured using elisa reader. the data were analyzed statistically using non-parametric kruskal-wallis test and mann-whitney test. 5% eep solution has the same antibacterial efficacy as 2% chlorhexidine against e. faecalis. antibacterial efficacy of 5% eep solusion is statistically equal to that of 2% chlorhexidine solution in eliminating e. faecalis.