Polymorphisms of tumor necrosis factor- α,transforming growth factor- β,and interleukin-10 in asthma associated with olive pollen sensitization

Sensitization to specific olive pollen-allergens (ole e 2 and 10) has been correlated with a clinical pattern of asthma. this study analyzes the association between several polymorphims of tnfa (g-308a,c-857t,and c-1031t),il10 (c-571a and a-1117g),and tgfb (c-509-t) and these sensitizations. these polymorphisms were genotyped by allelic discrimination,in olive pollen-allergic patients (phenotyped for specific ole e 2 and 10 sensitizations) and healthy controls. levels of serum-soluble cytokines were correlated with specific genotypes and clinical phenotypes. the results showed that heterozygous tgfb c-509t genotype,besides having the lowest sera tgf- levels,was significantly increased in olive pollen-allergic patients compared with controls. according specific sensitizations,cc genotype of il10 c-571a could be a protective factor for ole e 2 sensitization and mainly for asthmatic ole e 2 sensitized patients compared with asthmatic non-ole e 2 sensitized patients (or: 0.26,p=0.008). in contrast,heterozygous ca genotype was increased in ole e 2 asthmatic subjects compared to asthmatic non-ole e 2 sensitized patients. lastly,heterozygous tnfa g-308a genotype was associated with ole e 10 sensitization (or: 2.5,p=0.04). in conclusion,these results suggest a role of tgf-β1 in olive-pollen sensitization and tnf-α and il-10 genotypes in the asthma induced by specific olive-pollen allergens. © 2014 blanca cárdaba et al.