Simultaneous detection and differentiation of highly virulent and classical Chinese-type isolation of PRRSV by real-time RT-PCR

Porcine reproductive and respiratory syndrome (prrs) is a leading disease in pig industry worldwide and can result in serious economic losses each year. the prrs epidemic situation in china has been very complicated since the unprecedented large-scale highly pathogenic prrs (hp-prrs) outbreaks in 2006. and now the hp-prrs virus (hp-prrsv) and classical north american type prrsv strains have coexisted in china. rapid differential detection of the two strains of prrsv is very important for effective prrs control. the real-time rt-pcr for simultaneous detection and differentiation of hp-prrsv and prrsv by using both sybr green and taqman probes was developed and validated. both assays can be used for rapid detection and strain-specific identification of hp-prrsv and prrsv. however,the taqman probe method had the highest detection rate whereas the conventional rt-pcr was the lowest. the real-time rt-pcr developed based on sybr green and taqman probe could be used for simultaneous detection and differentiation of hp-prrsv and prrsv in china,which will benefit much the prrs control and research. © 2014 shuqi xiao et al.