Hsp74,a potential bladder cancer marker,has direct interaction with keratin 1

Early diagnosis and prognosis monitoring are very important for the survival of patients with bladder cancer. to identify candidate biomarkers of bladder cancer,we used a combination of techniques including 2-de,co-ip,western blot,lc-ms/ms,and immunohistochemistry. hsp74 was identified with high expression in bladder cancer. the cellular location of expression products of gene hsp74 showed that they were distributed into cytoplasm and keratin 1 was found to be associated with hsp74. the results provide a new idea to understand the molecular basis of bladder cancer progression and pinpoint new potential molecular target for early diagnosis and therapeutic monitoring of bladder cancer. © 2014 ling chen et al.