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Fusion-expressed CTB improves both systemic and mucosal T-cell responses elicited by an intranasal DNA priming/intramuscular recombinant vaccinia boosting regimen
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نویسنده
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qiu s. ,ren x. ,ben y. ,ren y. ,wang j. ,zhang x. ,wan y. ,xu j.
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منبع
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journal of immunology research - 2014 - دوره : 2014 - شماره : 0
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چکیده
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Previous study showed that ctb (cholera toxin subunit b) can be used as a genetic adjuvant to enhance the systemic immune responses. to further investigate whether it can also be used as a genetic adjuvant to improve mucosal immune responses,we constructed dna and recombinant tiantan vaccinia (rttv) vaccines expressing ova-ctb fusion antigen. female c57bl/6 mice were immunized with an intranasal dna priming/intramuscular rttv boosting regimen. ova specific t-cell responses were measured by ifn-γ elispot and specific antibody responses were determined by elisa. compared to the nonadjuvant group (psv-ova intranasal priming/rttv-ova intramuscular boosting),psv-ova-ctb intranasal priming/rttv-ova-ctb intramuscular boosting group significantly improved the magnitudes of t-cell responses at spleen (1562±567 sfcs/106 splenocytes versus 330±182 sfcs/106 splenocytes,p<0.01),mesenteric ln (96±83 sfcs/106 lymphocytes versus 1±2 sfcs/106 lymphocytes,p<0.05),draining lns of respiratory tract (109±60 sfcs/106 lymphocytes versus 2±2 sfcs/10 6 lymphocytes,p<0.01) and female genital tract (89±48 sfcs/106 lymphocytes versus 23±21 sfcs/106 lymphocytes,p<0.01). these results collectively demonstrated that fusion-expressed ctb could act as a potent adjuvant to improve both systemic and mucosal t-cell responses. © 2014 sugan qiu et al.
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آدرس
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shanghai public health clinical center,fudan university, China, shanghai public health clinical center,fudan university, China, shanghai public health clinical center,fudan university, China, shanghai public health clinical center,fudan university, China, shanghai public health clinical center,fudan university,shanghai 201508,china,institute of biomedical science,fudan university, China, shanghai public health clinical center,fudan university,shanghai 201508,china,institute of biomedical science,fudan university, China, shanghai public health clinical center,fudan university, China, shanghai public health clinical center,fudan university,shanghai 201508,china,institute of biomedical science,fudan university, China
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Authors
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