Distribution of HLA-A,-B,and -C alleles and HLA/KIR combinations in han population in China

We investigated polymorphisms of the human leukocyte antigen (hla) class i (a,b,and c) loci of a han population (n,239) from the yunnan province,southwest china,using high-resolution polymerase chain reaction-luminex (pcr-luminex) typing. we combined the hla data from this study with the kir genotypes from a previous study of this han population to analyze the combination of kir/hla ligands. a total of 27 hla-a,54 hla-b,and 31 hla-c alleles were found in this population. the frequencies of a11:01,a24:02,b40:01,b46:01,c01:02,c03:04,and c07:02 were all > 10%. the following haplotypes were common,with frequencies > 5%: 1 a-b (a02:07-b46:01),2 a-c (a02:07-c01:02,and a11:01-c07:02),4 c-b (b13:01-c03:04,b40:01-c07:02,b46:01-c01:02 and b58:01-c03:02),and 1 a-c-b (a02:07-c01:02-b46:01). analysis of kir3d and their ligands hla-a3/a11 and hla-bw4 showed that the frequencies of 3dl2+-a3/a11+ and 3dl2+-a3/a11- were 0.527 and 0.473,and the frequencies of 3dl1+-bw4+,3dl1+-bw4-,3dl1--bw4+,and 3dl1--bw4- were 0.552,0.397,0.038,and 0.013,respectively. the results of kir/hla-c combination analysis showed that all individuals had at least one inhibitory or activating kir/hla-c pair,and one kir/hla-c pair was the most frequent (157/239),followed by two pairs (46/239),three pairs (33/239),and no pairs (3/239). comparison of kir gene and hla gene and their pair frequency between yunnan han and the isolated han (fydh) who also lived in yunnan province showed no significant difference (p>0.05) in kir frequencies,but significant differences (p<0.05) for some hla allele frequencies. in addition,there was no significant difference (p>0.05) between the two populations for kir/hla pairs. © 2014 yunsong shen et al.