|
|
|
|
Branched Polyethylenimine-Superparamagnetic Iron Oxide Nanoparticles (bPEI-SPIONs) Improve the Immunogenicity of Tumor Antigens and Enhance Th1 Polarization of Dendritic Cells
|
|
|
|
|
|
|
|
نویسنده
|
hoang m.-d. ,lee h.-j. ,lee h.-j. ,jung s.-h. ,choi n.-r. ,vo m.-c. ,nguyen-pham t.-n. ,kim h.-j. ,park i.-k. ,lee j.-j.
|
|
منبع
|
journal of immunology research - 2015 - دوره : 2015 - شماره : 0
|
|
چکیده
|
Nanoparticles in the field of dendritic cell (dc) research are emerging as a promising method of enhancing the efficacy of cancer immunotherapy. we investigated the effect of branched polyethylenimine-superparamagnetic iron oxide nanoparticles (bpei-spions) on tumor cells loaded onto dcs. the tumor antigens were prepared as follows: (1) apoptotic u266 cells with ultraviolet b (uvb) irradiation followed by a 2 h incubation in the absence (2 h postirradiated cells) or (2) presence of bpei-spions (bpei-spion 2 h postirradiated cells) and (3) apoptotic u266 cells with uvb irradiation followed by an overnight 16 h incubation (16 h postirradiated cells). bpei-spions render u266 cells sensitive to uvb irradiation through reactive oxygen species production to accelerate apoptotic death. the 2 h postirradiated cells and bpei-spion 2 h postirradiated cells released immunogenic proteins,including hsp70,hsp90,and hmgb1. the dcs loaded with bpei-spion 2 h postirradiated cells showed the highest il-12p70 production and th1 polarization compared with other dcs. these results suggest that bpei-spions are a promising method of enhancing the immunogenicity of tumor cells and promoting th1 polarization of dcs loaded with these tumor cells. © 2015 my-dung hoang et al.
|
|
|
|
|
آدرس
|
research center for cancer immunotherapy,chonnam national university,hwasun hospital,hwasun,jeollanamdo,south korea,department of hematology-oncology,chonnam national university,hwasun hospital,hwasun, South Korea, department of biomedical science,chonnam national university,medical school, South Korea, research center for cancer immunotherapy,chonnam national university,hwasun hospital,hwasun,jeollanamdo,south korea,department of hematology-oncology,chonnam national university,hwasun hospital,hwasun, South Korea, research center for cancer immunotherapy,chonnam national university,hwasun hospital,hwasun,jeollanamdo,south korea,department of hematology-oncology,chonnam national university,hwasun hospital,hwasun, South Korea, research center for cancer immunotherapy,chonnam national university,hwasun hospital,hwasun,jeollanamdo,south korea,department of hematology-oncology,chonnam national university,hwasun hospital,hwasun, South Korea, research center for cancer immunotherapy,chonnam national university,hwasun hospital,hwasun,jeollanamdo,south korea,department of hematology-oncology,chonnam national university,hwasun hospital,hwasun, South Korea, research center for cancer immunotherapy,chonnam national university,hwasun hospital,hwasun,jeollanamdo,south korea,department of hematology-oncology,chonnam national university,hwasun hospital,hwasun, South Korea, department of hematology-oncology,chonnam national university,hwasun hospital,hwasun, South Korea, department of biomedical science,chonnam national university,medical school, South Korea, research center for cancer immunotherapy,chonnam national university,hwasun hospital,hwasun,jeollanamdo,south korea,department of hematology-oncology,chonnam national university,hwasun hospital,hwasun, South Korea
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Authors
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|