Chlorophytum borivilianum Polysaccharide Fraction Provokes the Immune Function and Disease Resistance of Labeo rohita against Aeromonas hydrophila

The present study aimed to investigate the effects of chlorophytum borivilianum polysaccharide (cbp),as a dietary supplement administered at varying concentrations with feed (basal diet),on various cytokine-related responses in labeo rohita fingerlings. immune parameters and immune-related gene expressions were measured at 3rd,4th,and 5th week after feeding. the results revealed that dietary administration of cbp at 0.2% and 0.4% for 4 weeks significantly upregulated serum lysozyme and phagocytic activity. complement c3 and respiratory burst activity (rba) were significantly higher after 4 weeks of cbp feeding. the immune related genes il-8,il-1β,tnf-α,and inos were downregulated (p<0.05) in groups with 0.2% and 0.4% cbp supplemented diets at week 4. expression of anti-inflammatory cytokines (il-10 and tgf-β) was also downregulated (p<0.5) after 4 weeks of feeding with 0.2% to 0.8% cbp. however,five weeks of cbp administration had no significant effect on immune gene expression,except tnf-α and il-8. fish fed with 0.4% cbp for 4 weeks showed maximum resistance against aeromonas hydrophila (73.3% survival) compared to control. from these results,we recommend that cbp administration at 0.4% for 4 weeks could effectively improve immune response and disease resistance in l. rohita. © 2015 sib sankar giri et al.