Enhanced cysteinyl-leukotriene type 1 receptor expression in T cells from house dust mite-allergic individuals following stimulation with der p

In order to determine the potential for allergen to modulate t cell expression of the cysltreceptor and responsiveness to leukotrienes,peripheral blood mononuclear cells from house dust mite-allergic or nonallergic individuals were incubated with d. pteronyssinus allergen (der p). baseline cysltexpression was similar in both groups of donors,but der p significantly enhanced cysltexpression in cd4+ and cd8+ t cells of only allergic individuals and induced enhanced responsiveness of cd4+ t cells to ltdin terms of calcium mobilisation. this effect was prevented by the cysltantagonist mk571. der p also induced il-4 and il-10 production,and neutralizing antibody to il-4 prevented both the enhanced cysltexpression and the enhanced responsiveness of t cells to ltdinduced by der p. in allergic individuals,der p also induced t cell proliferation and a th2-biased phenotype. our data suggest that,in allergen-sensitized individuals,exposure to allergen can enhance t cell expression of cysltreceptors through a mechanism involving il-4 production. this,in turn,would induce cd4+ t cell responsiveness to cysteinyl-leukotrienes and th2 cell activation. © 2015 maryse thivierge et al.