Stimulation of Innate and Adaptive Immunity by Using Filamentous Bacteriophage fd Targeted to DEC-205

The filamentous bacteriophage fd,codisplaying antigenic determinants and a single chain antibody fragment directed against the dendritic cell receptor dec-205,is a promising vaccine candidate for its safety and its ability to elicit innate and adaptive immune response in absence of adjuvants. by using a system vaccinology approach based on rna-sequencing (rna-seq) analysis,we describe a relevant gene modulation in dendritic cells pulsed with anti-dec-205 bacteriophages fd. rna-seq data analysis indicates that the bacteriophage fd virions are sensed as a pathogen by dendritic cells; they activate the danger receptors that trigger an innate immune response and thus confer a strong adjuvanticity that is needed to obtain a long-lasting adaptive immune response. © 2015 luciana d'apice et al.