Low-Dose oxygen enhances macrophage-derived bacterial clearance following cigarette smoke exposure

Background. chronic obstructive pulmonary disease (copd) is a common,smoking-related lung disease. patients with copd frequently suffer disease exacerbations induced by bacterial respiratory infections,suggestive of impaired innate immunity. low-dose oxygen is a mainstay of therapy during copd exacerbations; yet we understand little about whether oxygen can modulate the effects of cigarette smoke on lung immunity. methods. wild-type mice were exposed to cigarette smoke for 5 weeks,followed by intratracheal instillation of pseudomonas aeruginosa (pao1) and 21% or 35-40% oxygen. after two days,lungs were harvested for pao1 cfus,and bronchoalveolar fluid was sampled for inflammatory markers. in culture,macrophages were exposed to cigarette smoke and oxygen (40%) for 24 hours and then incubated with pao1,followed by quantification of bacterial phagocytosis and inflammatory markers. results. mice exposed to 35-40% oxygen after cigarette smoke and pao1 had improved survival and reduced lung cfus and inflammation. macrophages from these mice expressed less tnf and more scavenger receptors. in culture,macrophages exposed to cigarette smoke and oxygen also demonstrated decreased tnf secretion and enhanced phagocytosis of pao1 bacteria. conclusions. our findings demonstrate a novel,protective role for low-dose oxygen following cigarette smoke and bacteria exposure that may be mediated by enhanced macrophage phagocytosis. © 2016 william g. bain et al.