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   Comparison of Intranasal Outer Membrane Vesicles with Cholera Toxin and Injected MF59C.1 as Adjuvants for Malaria Transmission Blocking Antigens AnAPN1 and Pfs48/45  
   
نویسنده pritsch m. ,ben-khaled n. ,chaloupka m. ,kobold s. ,berens-riha n. ,peter a. ,liegl g. ,schubert s. ,hoelscher m. ,l�scher t. ,wieser a.
منبع journal of immunology research - 2016 - دوره : 2016 - شماره : 0
چکیده    Purified protein vaccines often require adjuvants for efficient stimulation of immune responses. there is no licensed mucosal adjuvant on the market to adequately boost the immune response to purified antigens for intranasal applications in humans. bacterial outer membrane vesicles (omv) are attractive candidates potentially combining antigenic and adjuvant properties in one substance. to more precisely characterize the potential of escherichia coli omv for intranasal vaccination with heterologous antigens,immune responses for anapn1 and pfs48/45 as well as ovalbumin as a reference antigen were assessed in mice. the intranasal adjuvant cholera toxin (ct) and parenteral adjuvant mf59c.1 were used in comparison. vaccinations were administered intranasally or subcutaneously. antibodies (total igg and igm as well as subclasses igg1,igg2a,igg2b,and igg3) were measured by elisa. t cell responses (cytotoxic t cells,th1,th17,and regulatory t cells) were determined by flow cytometry. when omv were used as adjuvant for intranasal immunization,antibody and cellular responses against all three antigens could be induced,comparable to cholera toxin and mf59c.1. antigen-specific igg titres above 1: 105 could be detected in all groups. this study provides the rationale for further development of omv as a vaccination strategy in malaria and other diseases. � 2016 michael pritsch et al.
آدرس division of infectious diseases and tropical medicine,medical center,university of munich (lmu),munich,germany,department of bacteriology,max von pettenkofer institute (lmu),munich,germany,german center for infection research (dzif),partner site munich,munich, Germany, division of infectious diseases and tropical medicine,medical center,university of munich (lmu),munich, Germany, center of integrated protein science munich (cips-m),division of clinical pharmacology,department of internal medicine iv,university of munich (lmu),munich,germany,german center for lung research,munich, Germany, center of integrated protein science munich (cips-m),division of clinical pharmacology,department of internal medicine iv,university of munich (lmu),munich,germany,german center for lung research,munich, Germany, division of infectious diseases and tropical medicine,medical center,university of munich (lmu),munich, Germany, department of bacteriology,max von pettenkofer institute (lmu),munich, Germany, department of bacteriology,max von pettenkofer institute (lmu),munich, Germany, department of bacteriology,max von pettenkofer institute (lmu),munich, Germany, division of infectious diseases and tropical medicine,medical center,university of munich (lmu),munich,germany,german center for infection research (dzif),partner site munich,munich, Germany, division of infectious diseases and tropical medicine,medical center,university of munich (lmu),munich, Germany, division of infectious diseases and tropical medicine,medical center,university of munich (lmu),munich,germany,department of bacteriology,max von pettenkofer institute (lmu),munich,germany,german center for infection research (dzif),partner site munich,munich,germany,college of public health and medical science,jimma university,jimma, Ethiopia
 
     
   
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