Release of Type 2 Cytokines by Epithelial Cells of Nasal Polyps

Background. t2 inflammation of chronic rhinosinusitis with nasal polyps (crswnp) may be influenced by epithelial cytokines release (tslp,il-25,and il-33). we investigated the release of tslp,il-25,and il-33 by epithelial crswnp cells compared to epithelial sinus mucosa cells of patients with chronic rhinosinusitis without nasal polyps (crssnp). methods. il-25,il-33,and tslp were measured by elisa in the supernatant of cell cultures derived by crswnp (9 patients,6 atopic) and crssnp (7 patients,2 atopic) in baseline condition and following stimulation with dermatophagoides pteronyssinus (dp),aspergillus fumigatus (af),and poly(i:c). results. crswnp epithelial cells released increased levels of il-25 (from 0.12 � 0.06 pg/ml to 0.27 � 0.1 pg/ml,p<0.01) and tslp (from 0.77 � 0.5 pg/ml to 2.53 � 1.17 pg/ml,p<0.001) following poly(i:c) stimulation,while crssnp epithelial cells released increased levels of il-25 and il-33 following af and dp stimulation,respectively (il-25: from 0.18 � 0.07 pg/ml to 0.51 � 0.1 pg/ml,p<0.001; il-33: from 2.57 � 1.3 pg/ml to 5.7 � 3.1 pg/ml,p<0.001). conclusions. crswnp epithelial cells release tslp and il-25 when stimulated by poly(i:c) but not by dp or af,suggesting that viral infection may contribute to maintain and amplify the t2 immune response seen in crswnp. � 2016 monica boita et al.