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   Structural and Functional Characterization of Recombinant Interleukin-10 from Indian Major Carp Labeo rohita  
   
نویسنده karan s. ,dash p. ,kaushik h. ,sahoo p.k. ,garg l.c. ,dixit a.
منبع journal of immunology research - 2016 - دوره : 2016 - شماره : 0
چکیده    Interleukin-10,an important regulator of both the innate and adaptive immune systems,is a multifunctional major cytokine. though it is one of the major cytokines,il-10 from the indian major carp,labeo rohita,has not yet been characterized. in the present study,we report large scale production and purification of biologically active recombinant il-10 of l. rohita (rlril-10) using a heterologous expression system and its biophysical and functional characterization. high yield (∼70 mg/l) of soluble rlril-10 was obtained at shake flask level. the rlril-10 was found to exist as a dimer. far-uv cd spectroscopy showed presence of predominantly alpha helices. the tertiary structure of the purified rlril-10 was verified by fluorescence spectroscopy. two-dimensional gel analysis revealed the presence of six isoforms of the rlril-10. the rlril-10 was biologically active and its administration significantly reduced serum proinflammatory cytokines,namely,interleukin 1β,tnf,and il-8,and augmented the nkef transcript levels in spleen of l. rohita. anti-inflammatory role of the rlril-10 was further established by inhibition of phagocytosis using nbt reduction assay in vitro. the data indicate that the dimeric alpha helical structure and function of il-10 of l. rohita as a key regulator of anti-inflammatory response have remained conserved during evolution. � 2016 sweta karan et al.
آدرس gene regulation laboratory,national institute of immunology,aruna asaf ali marg,new delhi,india,school of biotechnology,jawaharlal nehru university,new delhi, India, fish health management division,central institute of freshwater aquaculture,bhubaneswar, India, gene regulation laboratory,national institute of immunology,aruna asaf ali marg,new delhi, India, fish health management division,central institute of freshwater aquaculture,bhubaneswar, India, gene regulation laboratory,national institute of immunology,aruna asaf ali marg,new delhi, India, school of biotechnology,jawaharlal nehru university,new delhi, India
 
     
   
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