Anti-Inflammatory Effects of Ginsenoside Rg3 via NF- B Pathway in A549 Cells and Human Asthmatic Lung Tissue

Objective. there is limited information of the anti-inflammatory effects of rg3 on inflamed lung cells and tissues. therefore,we confirmed the anti-inflammatory mechanism of ginsenoside rg3 in inflamed human airway epithelial cells (a549) and tissues whether rg3 regulates nuclear factor kappa b (nf-b) activity. methods. to induce the inflammation,il-1β (10 ng/ml) was treated to a549 cells for 4 h. the effects of rg3 on nf-b activity and cox-2 expression were evaluated by western blotting analysis in both il-1β-induced inflamed a549 cell and human asthmatic airway epithelial tissues. using multiplex cytokines assay,the secretion levels of nf-b-mediated cytokines/chemokines were measured. result. rg3 showed the significant inhibition of nf-b activity thereby reduced cox-2 expression was determined in both il-1β-induced inflamed a549 cell and human asthmatic airway epithelial tissues. in addition,among nf-b-mediated cytokines,the secretion levels of il-4,tnf-α,and eotaxin were significantly decreased by rg3 in asthma tissues. even though there was no significant difference,il-6,il-9,and il-13 secretion showed a lower tendency compared to saline-treated human asthmatic airway epithelial tissues. conclusion. the results from this study demonstrate the potential of rg3 as an anti-inflammatory agent through regulating nf-b activity and reducing the secretion of nf-b-mediated cytokines/chemokines. © 2016 in-seung lee et al.