LPS Cooperates with Poly-L-Arginine to Promote IL-6 and IL-8 Release via the JNK Signaling Pathway in NCI-H292 Cells

Objective. herein,we aimed to study the mechanism whereby poly-l-arginine (pla) and lipopolysaccharide (lps) can synergistically induce the release of interleukin-6 (il-6) and il-8 in nci-h292 cells. methods. nci-h292 cells were divided into control,pla,lps,and pla+lps groups. at various time points,the phosphorylation of jnk in each group was measured by western blotting. additionally,the productions of il-6 and il-8 were assessed using an enzyme-linked immunosorbent assay (elisa). the effects of sp600125,an inhibitor of the jnk pathway,on the increase of p-jnk,il-6,and il-8 were also studied. results. our results showed that either pla or lps treatment alone can significantly increase the phosphorylation level of jnk in nci-h292 cells. of interest was the combined use of pla and lps that has a synergistic effect on the phosphorylation of jnk,as well as synergistically inducing the release of il-6 and il-8 in nci-h292 cells. furthermore,sp600125 significantly inhibited the activation of jnk signal,as well as reducing the productions of il-6 and il-8 in response to pla+lps stimulation. conclusions. the jnk signaling pathway contributes to the release of il-6 and il-8,which is stimulated by the synergistic actions of pla+lps in nci-h292 cells. � 2016 ling-ling zhang et al.