Gene Expression Profiles of Human Phosphotyrosine Phosphatases Consequent to Th1 Polarisation and Effector Function

Phosphotyrosine phosphatases (ptps) constitute a complex family of enzymes that control the balance of intracellular phosphorylation levels to allow cell responses while avoiding the development of diseases. despite the relevance of cd4 t cell polarisation and effector function in human autoimmune diseases,the expression profile of ptps during t helper polarisation and restimulation at inflammatory sites has not been assessed. here,a systematic analysis of the expression profile of ptps has been carried out during th1-polarising conditions and upon pkc activation and intracellular raise of ca2+ in effector cells. changes in gene expression levels suggest a previously nonnoted regulatory role of several ptps in th1 polarisation and effector function. a substantial change in the spatial compartmentalisation of erk during t cell responses is proposed based on changes in the dose of cytoplasmic and nuclear mapk phosphatases. our study also suggests a regulatory role of autoimmune-related ptps in controlling t helper polarisation in humans. we expect that those ptps that regulate t helper polarisation will constitute potential targets for intervening cd4 t cell immune responses in order to generate new therapies for the treatment of autoimmune diseases. copyright © 2017 patricia castro-sánchez et al.