Glycomacropeptide Attenuates Inflammation,Pruritus,and Th2 Response Associated with Atopic Dermatitis Induced by 2,4-Dinitrochlorobenzene in Rat

Atopic dermatitis (ad) is one of the most common skin diseases,whose incidence is increasing in industrialized countries. the epicutaneous application of a hapten,such as 2,4-dinitrochlorobenzene (dncb),evokes an experimental murine ad-like reaction. glycomacropeptide (gmp) is a dairy bioactive peptide derived from hydrolysis of -casein by chymosin action. it has anti-inflammatory,prebiotic,and immunomodulatory effects. the present study was aimed to investigate the effect of gmp administration on dncb-induced ad in rats. the severity of inflammatory process,pruritus,production of cytokines,and total immunoglobulin e (ige) content were measured,and the histopathological features were analyzed. gmp reduced the intensity of inflammatory process and edema of dncb-induced dermatitis,with a significant decrease in eosinophils recruitment and mast cells hyperplasia. in addition gmp suppressed the serum levels of total ige and il-4,il-5,and il-13 expression in ad-lesions. besides,the levels of il-10 were significantly increased. remarkably,gmp administration before ad-induction abolished pruritus in dermatitis-like reactions in the rats. taken together,these results indicate that gmp has an inhibitory effect on ad by downregulating th2 dominant immune response,suggesting gmp as a potential effective alternative therapy for the prevention and management of ad. © 2017 fabiola carolina muñoz et al.